A method utilizing zebrafish embryos to evaluate the toxicity and potential to cause birth defects of various substances. It involves exposing zebrafish embryos to test substances and observing their development for signs of toxicity or abnormalities.
Chorioallantoic Membrane Assay (CAM Assay)/Anti-angiogenic Test
A test that assesses the anti-angiogenic properties of substances using the chorioallantoic membrane (CAM) of fertilized chicken eggs. It involves applying the substance to the CAM and observing its effect on blood vessel formation, providing insights into its potential to inhibit angiogenesis, a process crucial for tumor growth and metastasis.
Brine Shrimp Lethality Assay
A simple bioassay used to screen the toxicity of substances. It involves exposing brine shrimp larvae (Artemia salina) to various concentrations of a substance and observing the mortality rate. This assay is quick, cost-effective, and widely used in preliminary toxicity screening of natural and synthetic compounds.