The DNA Barcoding Laboratory (DBL) aims to generate DNA barcodes in order to populate the Philippine faunal database. As of 2014, DBL has already generated DNA barcodes from 83 species from the Philippines, which include birds, fishes, snails, and nematodes, with more barcodes expected in the coming years. DBL also elucidates phylogenetic relationships of Philippine endemic taxa using molecular markers other than the DNA barcode sequences. DBL was established in 2008 and received and continues to receive funding support from the Office of the Vice Chancellor for Research and Development of UP Diliman, the Office of the Vice President for Academic Affairs and the Center for Integrative and Developmental Studies of the UP System, the Department of Science and Technology, Commission on Higher Education, and the Energy Development Corporation. Since 2008, DBL has already published 37 papers from the works of many graduate and undergraduate thesis students.
Ian Kendrich C. Fontanilla, Ph.D. (Group Head) Professor 5, UP Scientist I Ph.D. Genetics Molecular Genetics and Phylogenetics, Malacology |
Adrian U. Luczon, Ph.D. Candidate Instructor 7 Molecular Phylogenetics and Population Genetics |
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Raffy Jay C. Fornillos, M.Sc. Instructor 7 Parasitology, Molecular Helminthology, Medical Malacology |
Ronniel dC. Pedales, M.Sc. Instructor 7 Insect Ecology, Taxonomy and Systematics |
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Edwin Miguel S. Anadon, M.Sc. Research Associate |
Rogel Victor D. Mendoza, M.Sc. Research Associate |
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John Gregor A. Roño, M.Sc. Research Associate |
Mariah Gieanne D. Collado, B.Sc. University Research Associate I |
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Jose Gerald G. Pediengco, B.Sc. University Research Associate I |
Amina C. Kunting, B.Sc. University Research Associate I |
Graduate Students:
MS Biology
Acebedo, Jason Carlo T.
Catipon, Aaron Vince T.
Del Rosario, Lorraine Joyce M.
Kunting, Amina C.
PhD Biology
Fornillos, Raffy Jay C.
Luczon, Adrian U.
Manalo, Daria
Undergraduate Students:
Banaria, Francesca Helene C.
Naval, Joshua B.
Simsuangco, Jazmin Marie V.
Uy, Gillian Therese V.
Work Benches
Computer and Printing Station
PCR Work Station
Specimen Storage
Centrifuge Machines
Development of Novel Detection Tools for the Integrated Surveillance and Monitoring of Helminthic Neglected Tropical Diseases (NTDs) of Mutual Public Health Importance in the Philippines and China (DOST-Philippines & MOST-China Joint Research Program)
An Integrative Diagnostic Approach in Detecting Schistosomiasis in Humans and Water Buffaloes in Situations of Varying Levels of Prevalence in the Philippines (Joint Special Programme for Research and Training in Tropical Diseases /World Health Organization-Western Pacific Regional Office (TDR/WHO-WPRO) Small Grants Scheme for Implementation Research in Infectious Diseases of Poverty)
Identification of High Risk Areas for Taenia solium active transmission in the Philippines(World Health Organization)
Elucidation of transmission dynamics and ecoepidemiology of human Taenia infections in selected endemic provinces in the Philippines using environmental DNA (eDNA) (UP-OVPAA-Enhanced Creative Work and Research Grant)
Population genetics of the critically endangered Philippine Cockatoo (Cacatua haematuropygia) using single nucleotide polymorphisms in mitochondrial and nuclear genes (UP System OVPAA-ECWRG)
Phylogenetic placement and divergence time of the subfamily Cosmininae (Diptera: Rhiiniidae) based on the first mitogenomes for Isomiya and Strongyloneura (UP Diliman OVCRD Outright Grant)
Port and Ballast Water Baselines using Ecological, Microbiological, and eDNA Approaches (DOST-PCIEERD)
The Food-Borne Helminthiases (FBH) Problem in the Philippines: Present Status, Response and Proposed Intervention (DOST-PCHRD and DOH AHEAD)
Phylogentic analysis and population genetics of Philippine fruit bats (Pteropodidae) (UP System OVPAA-ECWRG)
Phylogeography and population genetics of the common short-nosed fruit bat Cynopterus brachyotis in the Philippines (UP Diliman OVCRD Outright Grant)
Defining Boundaries: Home Range, Habitat Use, and Genetic Diversity of the Philippine Tarsiers in the Mount Matutum Protected Landscape (Protect Wildlife, a USAID funded project)
Leonardo L, Hernandez L, Magturo TC, Palasi W, Rubite JM, de Cadiz A, Moendeg K, Fornillos R, Tabios IK, Mistica M, Fontanilla IKC. 2019. Current status of neglected tropical diseases (NTDs) in the Philippines. Acta Tropica doi.org/10.1016/j.actatropica.2019.105284.
Fornillos RJC, Sato MO, Tabios IKB, Sato M, Leonardo LR, Chigusa Y, Minamoto T, Kikuchi M, Legasi ER, Fontanilla IKC. 2019. Detection of Schistosoma japonicum and Oncomelania hupensis quadrasi environmental DNA and its potential utility to schistosomiasis japonica surveillance in the Philippines. PLoS ONE (14(11): e0224617.
Mendoza RVD, Fontanilla IKC. 2019. Whole mitochondrial genome of a Geoffroy’s Rousette, Rousettus amplexicaudatus (Pteropodidae). Mitochondrial DNA B 4(2): 3546-3548.
Calata FIC, Caranguian CZ, Mendoza JEM, Fornillos RJC, Tabios IKB, Fontanilla IKC, Leonardo LR, Sunico LS, Kawai S, Chigusa Y, Kikuchi M, Sato M, Toshifumi M, Baoanan ZG, Sato MO. 2019. Analysis of environmental DNA and edaphic factors for the detection of the snail intermediate host Oncomelania hupensis quadrasi. Pathogens 8: 160.
Batomalaque GA, Que GCL, Atong TAB, Masanga ARL, de Chavez ERC, Fontanilla IKC. 2019. Cytochrome c oxidase subunit 1 (COI) profile of the Philippine Helicostylinae (Gastropoda: Stylommatophora: Camaenidae). Philippine Journal of Science 148(S1): 1-13.
Luczon AU, Ampo SAMM, Rono JGA, Duya MRM, Ong PS, Fontanilla IKC. 2019. DNA barcodes reveal high genetic diversity in Philippine fruit bats. Philippine Journal of Science 148(S1): 133-140.
AMD II, Fontanilla IKC. 2019. Complete mitochondrial genome and novel gene organization of Ryssota otaheitana (Pulmonata: Chronidae) and its implications on the Stylommatophora phylogeny. Philippine Journal of Science 148(S1): 167-180.
Canoy RJC, Fontanilla IKC, Quilang JP. 2019. Molecular phylogeny of Philippine tigerperches (Perciformes, Terapontidae) based on mitochondrial genes. Philippine Journal of Science 148(S1): 251-261.
Ong PS, Padilla VJB, Serafica MPB, Luczon AU, Salvador DJI, Duya MV, Fontanilla IKC, Quilang JP. 2019. Development of microsatellites for the Philippine Eagle, Pithecophaga jefferyi, using Next Generation Sequencing. Philippine Journal of Science 148(S1): 263-266.
Fornillos RJC, Fontanilla IKC, Chigusa Y, Kikuchi M, Kirinoki M, Kato-Hayashi N, Kawazu S, Angeles JM, Tabios IK, Moendeg K, Goto Y, Tamayo PG, Gampoy EF, Pates I, Chua JC and Leonardo LR. 2019. Infection rate of Schistosoma japonicum in the snail Oncomelania hupensis quadrasi in endemic villages in the Philippines: Need for snail surveillance technique. Tropical Biomedicine, 36, 402–411.
Sato MO, Adsakwattana P, Fontanilla IKC, Kobayashi J, Sato M, Pongvongsa T, Fornillos RFC. 2019. Odds, challenges and new approaches in the control of helminthiasis, an Asian study. Parasite Epidemiology and Control, 4, e00083.
Pedales RDC, Fontanilla IKC. 2018. Forensic entomology in the Philippines : Establishing Baseline Data on the Forensically Important Blow Fly Species Chrysomya megacephala (Fabricius, 1794). Philippine Journal of Science, 147(1), 17–25.
Licuanan AM, Duya MRM, Ong PS, Fontanilla IKC. 2017. DNA barcoding, population genetics, and phylogenetics of the illegally hunted Philippine Duck Anas luzonica (Aves: Anseriformes: Anatidae). Journal of Threatened Taxa 9(5): 10141–10150.
Dumilag RV, Aguinaldo Z-ZA, Mintu CB, Quinto MB, Ame CE, Andres RC, Yap SL, Cao EP, Vital PG, Fontanilla IKC, Monotilla WD. 2017. A review of the current taxonomic status of foliose Bangiales (Rhodophyta) in the Philippines. Phytotaxa 312(1): 47-59.
Chua JC, Fontanilla IKC, Tabios IKB, Tamayo PG, De Chavez ERC, Agatsuma T, Kikuchi M, Kato-Hayashi N, Chigusa Y, Fornillos RJC, Leonardo LR. 2017. Genetic comparison of Oncomelania hupensis quadrasi (Mollendorf, 1895) (Gastropoda: Pomatiopsidae), the intermediate host of Schistosoma japonicum in the Philippines based on 16S ribosomal RNA sequence. Science Diliman 29(2): 32-50.
Moendeg KJ, Angeles JMM, Nakao R, Leonardo LR, Fontanilla IKC, Goto Y, Kirinoki M, Villacorte EA, Rivera PT, Inoue N, Chigusa Y, Kawazu S. 2017. Geographic strain differentiation of Schistosoma japonicum in the Philippines using microsatellite markers. PLoS Neglected Tropical Diseases 11(7): e0005749.
Fontanilla IKC, Naggs F, Wade CM. 2017. Molecular phylogeny of the Achatinoidea (Mollusca: Gastropoda). Molecular Phylogenetics and Evolution 114: 382-385.
Tujan MAA, Fontanilla IKC, Paller VGV. 2016. Vectors and spatial patterns of Angiostrongylus cantonensis in selected rice-farming villages of Muñoz, Nueva Ecija, Philippines. Journal of Parasitology Research 2016: 3085639.
Luczon AU, Ong PS, Quilang JP, Fontanilla IKC. 2016. Determining species identity from confiscated pangolin remains using DNA barcoding. Mitochondrial DNA Part B: Resources(1): 763-766.
Leonardo L, Chigusa Y, Kikuchi M, Kato-Hayashi N, Kawazu S, Angeles JM, Fontanilla IKC, Tabios IK, Moendeg K, Goto Y, Fornillos RJ, Tamayo PG, Chua JC. 2016. Schistosomiasis in the Philippines: challenges and some success in control. Southeast Asian Journal of Tropical Medicine and Public Health 47(4): 651-666.
Sagun JH, Davies KA, Fontanilla IKC, Chan MA, Laurente DA. 2015. Characterization of an isolate of Heterorhabditis bacteriophora (Nematoda: Heterorhabditidae) from the Northern Territory, Australia, using morphology and molecular data. Zootaxa.
De Chavez ERC, Fontanilla IKC, Batomalaque GA, Chiba S. 2015. A new Helicostyla species (Bradybaenidae: Helicostylinae) from Patnanungan Island, Philippines. Asia Life Sciences 24(1): 37-49.
Constantino-Santos DMA, Basiao ZU, Wade CM, Santos BS, Fontanilla IKC. 2014. Identification of Angiostrongylus cantonensis and other nematodes using the SSU rDNA in Achatina fulica populations of Metro Manila. Tropical Biomedicine 31(2): 327-335.
Constantino-Santos DMA, Santos BS, Soriano JME, Dy JSH, Fontanilla IKC. 2014. Philippine Survey of Nematode Parasite Infection and Load in the Giant African SnailAchatina fulica indicate Angiostrongylus cantonensis infection in Mindanao. Science Diliman 26(2): 72-84
Embrador MDM, Gan AGDC, Guanzon CMSM, Romilla CJM, Luber RP, Fontanilla IKC. 2014. Testing the utility of two mitochondrial genes (Cytochrome c oxidase I and 16S rRNA) as DNA barcodes for the family Muricidae (Gastropoda, Mollusca). The Philippine BIOTA 46:37-46
Fontanilla IKC, Sta Maria IMP, Garcia JRM, Ghate H, Naggs F, Wade CM. 2014. Restricted genetic variation in populations of Achatina (Lissachatina) fulica outside of East Africa and the Indian Ocean islands points to the Indian Ocean islands as the earliest known common source. PLoS ONE 9(9): e105151
Fontanilla IKC, Torres AF, Cañasa JADG, Yap SL, Ong PS. 2014. State of animal DNA barcoding in the Philippines: a review of COI sequencing of Philippine native fauna. Philippine Science Letters 7(1): 104-137.
Luczon AU, Fontanilla IKC, Ong PS, Basiao ZU, Sumaya AMT, Quilang JP. 2014. Genetic diversity of the critically endangered Philippine Eagle Pithecophaga jefferyi (Aves: Accipitridae) and notes on its conservation. Journal of Threatened Taxa 6(10): 6335-6344.
Batomalaque, G.A., Sales, P.R.L. and Fontanilla, I.K.C. 2013. DNA barcoding using cytochrome oxidase I (COI) of the pulmonate gastropods from Batan Island, Batanes, Philippines. Asia Life Sciences 22(2): 341-357.
Luczon, A.U., Torres, A.F., Quilang, J.P., Ong, P.S. and Fontanilla, I.K.C. 2013. DNA barcoding of birds in the University of the Philippines Diliman campus, with emphasis on striated grassbirds Megalurus palustris. Philippine Journal of Science 142(1): 1-11 .
Fontanilla, I.K.C. & Wade, C.M. 2012. First report of Angiostrongylus cantonensis in the Giant African Land Snail Achatina fulica in French Polynesia detected using the SSU rRNA gene. Tropical Biomedicine 29(4): 642-645.
Ong, P.S., Luczon, A.U., Quilang, J.P., Sumaya, A.M.T., Ibañez, J.C., Salvador, D.J. and Fontanilla, I.K.C. 2011. DNA barcodes of Philippine accipitrids. Molecular Ecology Resources 11: 245-254.
Aquilino, S.V.L., Tango, J.M., Fontanilla, I.K.C., Pagulayan, R.C., Basiao, Z.U., Ong, P.S. and Quilang, J.P. 2011. DNA barcoding of the ichthyofauna of Taal Lake, Philippines. Molecular Ecology Resources 11: 612-619.
Santos BS and Quilang JP. 2011. DNA barcoding of Arius species (Siluriformes: Ariidae) in Laguna de Bay, Philippines using the cytochrome c oxidase subunit I gene. Philippine Agricultural Scientist 94: 42-47.
Quilang JP, Santos BS, Ong PS, Basiao ZU, Fontanilla IKC, Cao EP. 2011. DNA barcoding of the Philippine endemic freshwater sardine Sardinella tawilis (Clupeiformes: Clupeidae) and its marine relatives. Philippine Agricultural Scientist 94(3): 16-25.
Aquino LM, Tango J, Canoy RJ, Fontanilla IKC, Basiao ZU, Ong PS, Quilang J. 2011. DNA barcoding of fishes of Laguna de Bay, Philippines. Mitochondrial DNA 22(4) 143-153.
Jumawan, J.C., Vallejo, B.M., Herrera, A.A., Buerano, C.C. and Fontanilla, I.K.C. 2011. DNA barcodes of the suckermouth sailfin catfish Pterygoplichthys (Siluriformes: Loricariidae) in the Marikina River system, Philippines: molecular perspective of an invasive alien fish species. Philippine Science Letters 4(2): 103-113.