Mammalian Cell Culture Service Lab
Laboratory Services
Sample Drying Services
This service can be availed for wet samples, e.g. plant extracts dissolved in extraction solvents such as ethanol, water, hexane, etc. Sample drying may be performed through either freeze-drying (lyophilization), rotary evaporation (rotavap), oven-drying at 40°C, or the combination of any 2 or all 3 methods (depending on the type, volume and characteristics of the sample). For the preparation of wet samples to be subjected to the bioassays listed below, this step is required.
DPPH Free Radical Scavenging Assay
A phytochemical assay which measures free radical scavenging capacity through reduction of the DPPH molecule. This service can be availed to assess whether your submitted sample has antioxidant properties. This service includes testing of 8 fixed concentrations of your sample, which are to be used to compute for an IC50 value.
Total Phenolics Content (TPC) Assay
A phytochemical assay which uses the Folin-Ciocalteu method to measure the concentration of total phenolic compounds in your submitted sample using gallic acid as standard.
Total Flavonoid Content (TFC) Assay
A phytochemical assay which uses the aluminum chloride method to measure the concentration of total flavonoid compounds in your submitted sample using quercetin as standard.
MTT Cytotoxicity Assay
A cell-based assay that is used to measure in vitro cellular metabolic activity though the reduction of the MTT molecule. This service can be availed to assess whether your submitted sample can induce changes in cell viability through proliferation or cytotoxic effects after 72 hours of incubation. This service includes testing of 8 fixed concentrations of your sample, which are to be used to compute for an IC50 value.
MTT Assay Photo-documentation
This is an additional service for the MTT assay wherein the cells treated with your submitted sample will be photographed under a phase-contrast microscope. This service can be availed to assess whether your submitted sample can induce changes in cell morphology.
Wound Healing/Scratch Assay
A cell-based assay that is used to measure collective cell migration by documenting the closure of a wound/scratch made on a cell monolayer over a period of 72 hours. This service can be availed to assess whether your submitted sample can induce changes in the rate of wound closure/cell migration. We require availing the MTT cytotoxicity assay prior to this service in order to establish the best concentration to test.
Annexin V-PI Apoptosis Assay
A cell-based assay that is used as a confirmatory step for an observed cytotoxic effect. This assay detects whether dying cells are undergoing apoptosis or necrosis by fluorescently tagging cells with Annexin V (green) and propidium iodide (PI) (red). We require availing the MTT cytotoxicity assay prior to this service in order to establish the best concentration to test.
Immunofluorescent Staining Assay
A cell-based assay that is used as a confirmatory step for an observed cellular effect. This assay detects the presence/absence and cellular localization of a particular antigen/protein of interest through the use of a fluorescently-conjugated antibody that specifically binds to that antigen/protein. For our service, we offer immunofluorescent staining of two well-studied proteins: Ki-67 (a cancer proliferation marker) and p53 (a tumor suppressor marker). We require availing the MTT cytotoxicity assay prior to this service in order to establish the best concentration to test.
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